First, an overview of ELISA testing
ELISA (enzyme-linked immunosorbent assay) is a highly sensitive experimental technique that combines the specific reaction of antigens and antibodies with the efficient catalytic action of enzymes on substrates based on immunological reactions. Since the reaction of the antigen and the antibody is carried out in the well of a solid phase carrier-polystyrene microtiter plate, after each reagent is added, the excess free reactant can be removed by washing to ensure the specificity of the test result. stability.
In practical applications, there are many specific method steps through different designs. Namely: an indirect method for detecting an antibody, a double antibody sandwich method for detecting an antigen, an antigen competition method for detecting a small molecule antigen or a hapten, and the like. At present, we provide customers with the commonly used ELISA double antibody sandwich method and ELISA indirect method service.
Second, the experimental technical process
1. Coating the antigen or antibody on a solid support
2, join the test specimen response
3. Add enzyme-labeled antigen or antibody
4, adding substrate catalytic enzyme color development
5, adding a stop solution to terminate the color reaction
6. Qualitative and quantitative analysis of antigens or antibodies by colorimetry
Third, the service process
1, pre-sales service, customers in the collection of specimens, technical advice, etc., the company's technical staff can first communicate.
2. The customer collects the specimen and expresses it to our company (Shanghai can pick up the specimen)
3, the laboratory conducts testing services
4. After the customer pays, the result of the test is sent to the customer by the salesperson.
5. If the customer receives the result, please contact the sales staff if you have any questions. The sales staff will inform the technical department to provide technical analysis and Q&A.
Fourth, the customer notice
1, liquid specimens: (cell culture supernatant, tissue homogenate, serum, plasma, urine, pleural effusion, ascites, cerebrospinal fluid, etc.)
2, sample size: each sample collection volume = (20-50ul) * number of detection indicators
3, sample preservation: long time must be frozen (-20 ° C or -70 ° C) to save. If you need to collect samples in a cycle, please pack the samples in time and store them at -20 °C or -70 °C.
4. Please tell us in advance: the species of your sample (human, rat, mouse, rabbit, pig, etc.), the number of samples, the indicators to be tested
5 When you send the specimen, please indicate the following:
1
Specimen number2
Tested item3
Whether to make a duplicate hole4
Contact information5
Whether the specimen is sent back after the experiment6, sample transportation: choose SF Express land transport or Zhongtong express land transport, sample preservation using insulation foam
Box, put dry ice or biological ice pack to keep it cool
V. Report content and standards
1, ELISA standard curve
2. Detailed experimental steps (see instructions)
3. Complete experimental report (experimental instrument, experimental results and results description)
Sixth, charging standards and service cycle
The charging standard and service period vary depending on the samples provided by the customer. For details, please contact the customer service specialist or refer to the signed cooperation contract.
Seven, quality assurance
Suitable for testing including serum, plasma, urine, cell culture supernatant, tissue homogenate, cell lysate and other specimens with extremely high sensitivity. Ensure that the results are accurate, objective, and credible, but there is no guarantee that a particular result will be obtained.
Eight, after-sales service
The company will conduct customer return visits from time to time to improve the quality of our services. This clause is based on the cooperation contract signed, and the details will vary depending on the contract.
The following is the detection data of human S100 calcium binding protein A8/A9 complex (S100A8/A9) provided by Jinma Life Science Research Center.
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